Vitamin D3 ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

Vitamin D3

Applications

Competitive ELISA

Reactivity

Universal

Assay type

Competitive (quantitative)

Sensitivity

0.94 ng/ml

Sample type

Serum, plasma and other biological fluids.

Cross reactivity

Specific for Vitamin D3 with no reported cross-reactivity with other analogs or variants.

Detection type

Colorimetric Detection (OD450)

Platform

Pre-coated Microplate (12 x 8 well strips)

Product range

1.56 - 100 ng/ml

Assay Time

2 hours

Principiul testului

Vitamin D3 ELISA Kit (A326975) employs the competitive enzyme immunoassay technique for the quantitative measurement of Vitamin D3 in serum, plasma, and other biological fluids. The 96-well microtiter plate has been pre-coated with Vitamin D3 antigen. During the incubation, Vitamin D3 present in the samples or standards competes with the fixed amount of immobilized Vitamin D3 for binding sites on the Biotinylated Anti-Vitamin D3 Antibody. The more Vitamin D3 present in a sample or standard, the less Biotinylated Anti-Vitamin D3 Antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-Vitamin D3 Antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of Vitamin D3 present in each sample or standard. The concentration of Vitamin D3 can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Storage

Shipped at +4°C. Store kit components at +4°C or -20°C as instructed in the Protocol Manual. Do not use past expiration date! Avoid freeze/thaw cycles.