Universal HRP Conjugated Streptavidin ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

HRP Conjugated Streptavidin

Applications

Direct ELISA

Reactivity

Universal

Assay type

Direct (quantitative)

Sensitivity

14 µIU/ml

Sample type

Serum, plasma and other biological fluids.

Cross reactivity

Specific for HRP Conjugated Streptavidin with no reported cross-reactivity with other analogs or variants.

Detection type

Colorimetric Detection (OD450)

Platform

Pre-coated Microplate (12 x 8 well strips)

Product range

28.125-1800 µIU/mL (1800 µU = 6 ng)

Assay Time

1 hour

Principiul testului

Universal HRP Conjugated Streptavidin ELISA Kit (A326620) is a 1 hour direct Enzyme-Linked Immunosorbent Assay (dELISA) designed for the in vitro quantitative determination of universal HRP Conjugated Streptavidin in serum, plasma, and other biological fluids. This assay directly measures HRP-SA activity through an HRP-mediated colorimetric reaction. TMB substrate is added and catalysed by HRP to produce a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is directly proportional to the amount of HRP Conjugated Streptavidin present in the sample. The concentration is calculated by reading the absorbance at 450 nm using a colorimetric microplate reader and comparing the values with the standard curve. The assay has a detection range of 28.125–1800 uIU/mL with a sensitivity of 14 uIU/mL.

Storage

Shipped at +4°C. Store kit components at +4°C or -20°C as instructed in the Protocol Manual. Do not use past expiration date! Avoid freeze/thaw cycles.