Imagine indisponibilăSheep HLA-DQB2 ELISA Kit
Pe scurt
Sheep HLA-DQB2 ELISA Kit este un kit ELISA de tip Sandwich (quantitative) pentru măsurarea HLA-DQB2, cu reactivitate declarată pentru Sheep. Măsoară în domeniul 0.313-20 ng/ml, are o sensibilitate de 0.188 ng/ml și folosește detecție Colorimetric Detection (OD450). Se livrează în mărimea 96T, la prețul de 4.492,13 lei cu TVA.
Produse destinate exclusiv cercetării. Nu se utilizează în diagnostic sau tratament.
Descriere
Sheep HLA-DQB2 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of sheep HLA-DQB2 in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.
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Specificații
- Brand
- Antibodies
- Mărime
- 96T
- Țintă
- HLA-DQB2
- Applications
- Sandwich ELISA
- Reactivity
- Sheep
- Assay type
- Sandwich (quantitative)
- Sensitivity
- 0.188 ng/ml
- Sample type
- Serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids.
- Cross reactivity
- Specific for HLA-DQB2 with no reported cross-reactivity with other analogs or variants.
- Detection type
- Colorimetric Detection (OD450)
- Product range
- 0.313-20 ng/ml
- Assay Time
- 4 hours
- Principiul testului
- Sheep HLA-DQB2 ELISA Kit (A326616) is a 4 hour sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of sheep HLA-DQB2 in serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids. A 96-well microtiter plate has been pre-coated with an antibody specific for HLA-DQB2. Standards and samples are added to the wells, where HLA-DQB2 binds to the immobilized capture antibody. Following incubation, unbound components are removed by washing and a biotinylated detection antibody specific for HLA-DQB2 is added to the wells. The detection antibody binds the captured HLA-DQB2 to form an antibody-antigen-antibody sandwich complex. After further incubation and washing, HRP-Streptavidin conjugate is added to the wells. TMB substrate solution is then added to visualize the HRP enzymatic reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is directly proportional to the amount of HLA-DQB2 captured in each well. The concentration of HLA-DQB2 is calculated by reading the absorbance at 450 nm and comparing the values with the standard curve.
Documentație
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