Chicken Estradiol ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

Estradiol

Applications

Competitive ELISA

Reactivity

Chicken

Recommended dilutions

Estimate the expected concentration of samples prior to the assay. Serum and plasma samples are recommended for use undiluted whenever possible. If sample dilution is required, use the sample diluent provided in the kit to maintain assay consistency.

Assay type

Competitive (quantitative)

Sensitivity

3.18 pg/ml

Sample type

Serum and plasma

Cross reactivity

Specific for Estradiol with no reported cross-reactivity with other analogs or variants.

Detection type

Colorimetric Detection (OD450)

Platform

Pre-coated Microplate (12 x 8 well strips)

Product range

7.81 - 500 pg/ml

Assay Time

1h 30m

Principiul testului

Chicken Estradiol ELISA Kit (A327133) employs a rapid competitive Enzyme-Linked Immunosorbent Assay (ELISA) technique for the quantitative measurement of chicken Estradiol in serum and plasma. Antigen specific for Estradiol has been pre-coated onto a 96-well microtiter plate. Standards and test samples are added to the wells together with HRP-conjugated detection reagent working solution. During incubation, Estradiol present in the sample competes with the immobilized antigen for binding to the HRP-conjugated detection reagent. Following incubation, unbound components are removed by washing. TMB substrate solution is then added to visualize the HRP enzymatic reaction, producing a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is inversely proportional to the concentration of Estradiol present in the sample. The concentration of Estradiol is determined by measuring the O.D. absorbance at 450 nm using a microplate reader and comparing the values to the standard curve.

Storage

Shipped at +4°C. Store kit components at +4°C as instructed in the Protocol Manual for up to 6 months. Do not use past expiration date!