Universal CPD (Cyclobutane Pyrimidine Dimers) ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

CPD (Cyclobutane Pyrimidine Dimers)

Applications

Competitive ELISA

Reactivity

Universal

Assay type

Competitive (quantitative)

Sensitivity

0.094 ng/ml

Sample type

Serum, plasma, cell culture supernatant, cell or tissue lysate, urine, and other biological fluids.

Cross reactivity

Specific for CPD (Cyclobutane Pyrimidine Dimers) with no reported cross-reactivity with other analogs or variants.

Detection type

Colorimetric Detection (OD450)

Platform

Pre-coated Microplate (12 x 8 well strips)

Product range

0.156-10 ng/ml

Assay Time

2 hours

Principiul testului

Universal CPD (Cyclobutane Pyrimidine Dimers) ELISA Kit (A326501) is a 2 hour competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of universal CPD (Cyclobutane Pyrimidine Dimers) in serum, plasma, cell culture supernatant, cell or tissue lysate, urine, and other biological fluids. A 96-well microtiter plate has been pre-coated with CPD (Cyclobutane Pyrimidine Dimers). During the assay, CPD (Cyclobutane Pyrimidine Dimers) present in the standards or samples competes with immobilized CPD (Cyclobutane Pyrimidine Dimers) for binding to an assay-specific antibody. Following incubation, unbound components are removed by washing and HRP-Streptavidin conjugate is added to the wells. After further incubation and washing, TMB substrate solution is added to visualize the HRP enzymatic reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the colour is measured at 450 nm using a microplate reader. The concentration of CPD (Cyclobutane Pyrimidine Dimers) in the samples is inversely proportional to the OD450 value and is determined by comparison with the standard curve.

Storage

Shipped at +4°C. Store kit components at +4°C or -20°C as instructed in the Protocol Manual. Do not use past expiration date! Avoid freeze/thaw cycles.

Documentație

• Manual de utilizare (PDF)se deschide pe site-ul producătorului