Imagine indisponibilăBovine C-Peptide ELISA Kit
Pe scurt
Bovine C-Peptide ELISA Kit este un kit ELISA de tip Sandwich (quantitative) pentru măsurarea C Peptide, cu reactivitate declarată pentru Bovine. Măsoară în domeniul 78.125-5000 pg/ml, are o sensibilitate de 46.875 pg/ml și folosește detecție Colorimetric Detection (OD450). Se livrează în mărimea 96T, la prețul de 4.957,98 lei cu TVA.
Produse destinate exclusiv cercetării. Nu se utilizează în diagnostic sau tratament.
Descriere
Bovine C-Peptide ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of bovine C-Peptide in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.
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Specificații
- Brand
- Antibodies
- Mărime
- 96T
- Țintă
- C Peptide
- Applications
- Sandwich ELISA
- Reactivity
- Bovine
- Assay type
- Sandwich (quantitative)
- Sensitivity
- 46.875 pg/ml
- Sample type
- Serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids.
- Cross reactivity
- Specific for C Peptide with no reported cross-reactivity with other analogs or variants.
- Detection type
- Colorimetric Detection (OD450)
- Product range
- 78.125-5000 pg/ml
- Assay Time
- 4 hours
- Principiul testului
- Bovine C-Peptide ELISA Kit (A326500) is a 4 hour sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of bovine C-Peptide in serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids. A 96-well microtiter plate has been pre-coated with an antibody specific for C-Peptide. Standards and samples are added to the wells, where C-Peptide binds to the immobilized capture antibody. Following incubation, unbound components are removed by washing and a biotinylated detection antibody specific for C-Peptide is added to the wells. The detection antibody binds the captured C-Peptide to form an antibody-antigen-antibody sandwich complex. After further incubation and washing, HRP-Streptavidin conjugate is added to the wells. TMB substrate solution is then added to visualize the HRP enzymatic reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is directly proportional to the amount of C-Peptide captured in each well. The concentration of C-Peptide is calculated by reading the absorbance at 450 nm and comparing the values with the standard curve.
Documentație
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