Human Hepatitis B Surface Antigen ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

Hepatitis B Surface Antigen

Applications

Sandwich ELISA

Reactivity

Human

Recommended dilutions

The suggestion is to use undiluted serum and plasma samples.

Assay type

Sandwich (quantitative)

Sensitivity

0.19 ng/ml

Sample type

Serum, plasma and other biological fluids.

Cross reactivity

Specific for Hepatitis B Surface Antigen with no reported cross-reactivity with other analogs or variants.

Detection type

Colorimetric Detection (OD450)

Platform

Pre-coated Microplate (12 x 8 well strips)

Product range

0.31-20 ng/ml

Assay Time

3h 30m

Principiul testului

Human Hepatitis B Surface Antigen ELISA Kit (A327025) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human Hepatitis B Surface Antigen in serum, plasma, and other biological fluids. An antibody specific for Hepatitis B Surface Antigen has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Hepatitis B Surface Antigen present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Hepatitis B Surface Antigen Antibody, which binds the captured Hepatitis B Surface Antigen present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Hepatitis B Surface Antigen captured in each well. The concentration of Hepatitis B Surface Antigen can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Storage

Shipped at +4°C. Store kit components at +4°C or -20°C as instructed in the Protocol Manual. Do not use past expiration date! Avoid freeze/thaw cycles.