Recombinant Human PDGF BB Protein

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Specificații

Brand

Antibodies

Mărime

10µg

Țintă

PDGF BB

Applications

ELISA, WB, Functional Assays

Formulation

Supplied in 30 mM Acetic Acid.

Product form

Lyophilized

Concentration

100 µg/ml after reconstitution.

Recommended dilutions

ELISA: 0.2 - 0.4ng/well, Functional Assays: 0.2ng/ml - 10ng/ml, WB: 1.5 - 3ng/lane

Purity

>98% (by SDS-PAGE and HPLC).

Reconstitution

Reconstitute with 0.1 ml of distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gently mix after reconstitution. After the initial reconstitution step, the product should be diluted to a working concentration using an aqueous buffer containg 0.1% Bovine Serum Albumin (or any albumin), or diluted in culture media containing serum, such as FCS.

Expression system

Escherichia coli

Nature

Recombinant

Protein species

Human

Endotoxin level

< 0.1 ng/µg

Specificitate

Recombinant Human PDGF BB homodimer is an E. coli-derived recombinant protein corresponding to mature full-length human Platelet-Derived Growth Factor BB homodimer (PDGF-BB), a potent mitogen for cells of mesenchymal origin. PDGF is composed of a dimer of two chains, known as the A chain and B chain. The dimer may present as either AA or BB homodimer, or as an AB heterodimer.Recombinant Human PDGF BB homodimer (PHP036) has an ED50 of 1.0-3.0 ng/ml as determined by stimulation of the proliferation of Balb/c 3T3 cells.

Storage

Lyophilized: Store at -20°C. Reconstituted: Store undiluted at -20°C, avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

Documentație

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