Rat Adiponectin ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

Adiponectin

Applications

Sandwich ELISA

Reactivity

Rat

Recommended dilutions

Estimate the expected concentration of samples prior to the assay. For serum and plasma samples, a 2-5 fold dilution is recommended using the sample diluent provided in the kit to maintain assay consistency.

Assay type

Sandwich (quantitative)

Sensitivity

6.43 ng/ml

Sample type

Serum and plasma

Cross reactivity

Specific for Adiponectin with no reported cross-reactivity with other analogs or variants.

Detection type

Colorimetric Detection (OD450)

Platform

Pre-coated Microplate (12 x 8 well strips)

Product range

15.63 - 1000 ng/ml

Assay Time

1h 30m

Principiul testului

Rat Adiponectin ELISA Kit (A327156) employs a rapid sandwich Enzyme-Linked Immunosorbent Assay (ELISA) technique for the quantitative measurement of rat Adiponectin in serum and plasma. An antibody specific for Adiponectin has been pre-coated onto a 96-well microtiter plate. Standards and test samples are added to the wells together with HRP-conjugated detection antibody working solution. During incubation, Adiponectin present in the sample binds to the immobilized capture antibody and forms a sandwich immune complex with the HRP-conjugated detection antibody. Following incubation, unbound components are removed by washing. TMB substrate solution is then added to visualize the HRP enzymatic reaction, producing a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is directly proportional to the amount of Adiponectin captured in each well. The concentration of Adiponectin is determined by measuring the O.D. absorbance at 450 nm using a microplate reader and comparing the values to the standard curve.

Storage

Shipped at +4°C. Store kit components at +4°C as instructed in the Protocol Manual for up to 6 months. Do not use past expiration date!