Mouse TIM 1 ELISA Kit (Small Sample Volume)

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Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

TIM-1

Applications

Sandwich ELISA

Reactivity

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

18.75 pg/ml

Sample type

Serum, plasma and other biological fluids.

Cross reactivity

Specific for TIM 1 with no reported cross-reactivity with other analogs or variants.

Detection type

Colorimetric Detection (OD450)

Platform

Pre-coated Microplate (12 x 8 well strips)

Product range

31.25 - 2000 pg/ml

Assay Time

3h 30m

Principiul testului

Mouse TIM 1 ELISA Kit (Small Sample Volume) (A323196) employs the sandwich Enzyme-Linked Immunoassay technique for the quantitative measurement of mouse in serum, plasma, and other biological fluids. This assay is optimized for limited sample availability, requiring only 25 µL of sample per well. An antibody specific for TIM 1 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the TIM 1 present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with biotinylated anti-TIM 1 antibody, which binds the captured TIM 1 present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Avidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of TIM 1 captured in each well. The concentration of TIM 1 can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Storage

Shipped at +4°C. Store kit components at +4°C or -20°C as instructed in the Protocol Manual. Do not use past expiration date! Avoid freeze/thaw cycles.