Anti-TIM 1 Antibody [RMT1-10] (PE)

Pe scurt

Descriere

Specificații

Brand

Antibodies

Mărime

100T

Țintă

TIM-1

Applications

Flow Cytometry

Reactivity

Mouse

Formulation

Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.

Conjugate

PE

Host

Rat

Clonality

Monoclonal

Product form

Lyophilized

Concentration

Reconstitution dependent.

Purification

Protein A affinity chromatography of tissue culture supernatant.

Isotype

IgG2a

Recommended dilutions

Flow Cytometry: Neat, Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl.

Reconstitution

Reconstitute with 1 ml of distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gently mix after reconstitution.

Clonă

RMT1-10

Imunogen

Full length mouse Tim-1-Ig, containing both the IgV and mucin domains of Tim-1.

Specificitate

This antibody recognises mouse Tim-1 (T-cell immunoglobulin mucin 1), a cell surface glycoprotein first identified as the hepatitis A virus cellular receptor (HAVCR). TIM-1 is highly expressed in the liver (isoform Tim-1a) and the kidneys (isoform Tim-1b).Tim-1 is highly expressed by activated CD4+ T cells, acting as a positive/negative co-stimulatory molecule of T cell proliferation, cytokine production and tolerance abrogation, and is the receptor for Tim-4, expressed on APCs. Tim-1 expression is greater on Th2 than Th1 cells, interaction between Tim-1 on Th2 cells and Tim-4 on dendritic cells (DCs), enhances Th2 cell function, and have implicated Tim-1 as a critical player in the development of atopic disease, and in particular airway hypersensitivity.In humans, polymorphic forms of Tim-1 are associated with increased susceptibility to asthma, eczema and rheumatoid arthritis. Rat anti Mouse TIM-1 antibody, clone RMT1-10 promotes Th2 responses, and inhibits antigen-specific T cell proliferation, in contrast to the agonistic function of many Tim-1 antibodies. Rat anti Mouse TIM-1 antibody, clone RMT1-10 reduces the severity of experimental autoimmune encephalomyelitis and delays disease onset in mice.

Storage

Lyophilized: Store at 4°C. Reconstituted: Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

Documentație

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