Mouse Proteinase-3 Anti-Neutrophil Cytoplasmic Antibody ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

Proteinase-3 Anti-Neutrophil Cytoplasmic Antibody

Applications

Sandwich ELISA

Reactivity

Mouse

Recommended dilutions

Serum and plasma samples may affect test results and should be diluted at least 1:2 with Sample Dilution Buffer prior to testing. Validated sample dilutions include Healthy serum (1:2) and Healthy plasma (1:2).

Assay type

Sandwich (quantitative)

Sensitivity

37.5 pg/ml

Sample type

Serum, plasma, tissue homogenates, and other biological fluids.

Cross reactivity

Demonstrates specificity for Proteinase-3 anti-Neutrophil Cytoplasmic antibodies with no reported cross-reactivity to related analogs.

Detection type

Colorimetric Detection (OD450)

Platform

Pre-coated Microplate (12 x 8 well strips)

Product range

62.5-4000 pg/ml

Assay Time

4 hours

Principiul testului

Mouse Proteinase-3 Anti-Neutrophil Cytoplasmic Antibody ELISA Kit (A303541) is a 4 hour sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse Proteinase-3 Anti-Neutrophil Cytoplasmic Antibody in serum, plasma, tissue homogenates, and other biological fluids. A 96-well microtiter plate has been pre-coated with assay-specific antigen. Standards and samples are added to the wells, where Proteinase-3 Anti-Neutrophil Cytoplasmic Antibody binds to the immobilized antigen. Following incubation, unbound components are removed by washing and a biotinylated antigen detection reagent is added to the wells. This reagent binds the captured Proteinase-3 Anti-Neutrophil Cytoplasmic Antibody to form an antigen-antibody-antigen sandwich complex. After further incubation and washing, HRP-Streptavidin conjugate is added to the wells. TMB substrate solution is then added to visualize the HRP enzymatic reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is directly proportional to the amount of Proteinase-3 Anti-Neutrophil Cytoplasmic Antibody captured in each well. The concentration of Proteinase-3 Anti-Neutrophil Cytoplasmic Antibody is calculated by reading the absorbance at 450 nm and comparing the values with the standard curve.

Storage

Shipped at +4°C. Store kit components at +4°C or -20°C as instructed in the Protocol Manual. Do not use past expiration date! Avoid freeze/thaw cycles.