Imagine indisponibilăMouse NQO1 ELISA Kit
Pe scurt
Mouse NQO1 ELISA Kit este un kit ELISA de tip Sandwich (quantitative) pentru măsurarea NQO1, cu reactivitate declarată pentru Mouse. Măsoară în domeniul 46.875-3000 pg/ml, are o sensibilitate de 28.125 pg/ml, folosește detecție Colorimetric Detection (OD450) și se poate folosi pe probe de tip Serum, plasma, tissue homogenates, and other biological fluids. Se livrează în mărimea 96T, la prețul de 4.591,95 lei cu TVA.
Produse destinate exclusiv cercetării. Nu se utilizează în diagnostic sau tratament.
Descriere
Mouse NQO1 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse NQO1 in serum, plasma, tissue homogenates, and other biological fluids.
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Specificații
- Brand
- Antibodies
- Mărime
- 96T
- Țintă
- NQO1
- Applications
- Sandwich ELISA
- Reactivity
- Mouse
- Recommended dilutions
- Validated sample dilutions include Mouse brain tissue lysate (1:2) and 4T1 cell lysate (undiluted).
- Assay type
- Sandwich (quantitative)
- Sensitivity
- 28.125 pg/ml
- Detection type
- Colorimetric Detection (OD450)
- Product range
- 46.875-3000 pg/ml
- Assay Time
- 4 hours
- Principiul testului
- Mouse NQO1 ELISA Kit (A303535) is a 4 hour sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse NQO1 in serum, plasma, tissue homogenates, and other biological fluids. A 96-well microtiter plate has been pre-coated with an antibody specific for NQO1. Standards and samples are added to the wells, where NQO1 binds to the immobilized capture antibody. Following incubation, unbound components are removed by washing and a biotinylated detection antibody specific for NQO1 is added to the wells. The detection antibody binds the captured NQO1 to form an antibody-antigen-antibody sandwich complex. After further incubation and washing, HRP-Streptavidin conjugate is added to the wells. TMB substrate solution is then added to visualize the HRP enzymatic reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is directly proportional to the amount of NQO1 captured in each well. The concentration of NQO1 is calculated by reading the absorbance at 450 nm and comparing the values with the standard curve.
Documentație
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