Mouse NGAL ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

NGAL

Applications

Sandwich ELISA

Reactivity

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

9.33 pg/ml

Sample type

Serum, plasma, cell culture supernates, cell lysates, tissue homogenates and other biological fluids.

Detection type

Colorimetric

Platform

Pre-coated Microplate (12 x 8 well strips)

Product range

78.1-5000 pg/ml

Assay Time

4 hours

Principiul testului

Mouse NGAL ELISA Kit (A119265) employs the sandwich enzyme immunoassay technique for the quantitative measurement of mouse NGAL in serum, plasma, cell culture supernates, cell lysates, tissue homogenates and other biological fluids. An antibody specific for NGAL has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the NGAL present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-NGAL Antibody, which binds the captured NGAL present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of NGAL captured in each well. The concentration of NGAL can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Storage

Shipped at 4°C. Unopened kits can be stored at +4°C for 12 months. Once opened products must be used within 1 month.

Documentație

• Manual de utilizare (PDF)se deschide pe site-ul producătorului