Mouse Fibrinogen alpha Chain ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărimi disponibile

48T · 96T

Țintă

Fibrinogen alpha Chain

Applications

Sandwich ELISA

Reactivity

Mouse

Assay type

Sandwich (quantitative)

Sensitivity

8.58 ng/ml

Sample type

Plasma.

Detection type

Colourmetric

Platform

Pre-coated Microplate (12 x 8 well strips)

Product range

31.2-2000 ng/ml

Assay Time

4h 30m

Principiul testului

Mouse Fibrinogen alpha Chain ELISA Kit (A3660) employs the sandwich enzyme immunoassay technique for the quantitative measurement of mouse Fibrinogen alpha Chain in plasma. An antibody specific for Fibrinogen alpha Chain has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Fibrinogen alpha Chain present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Fibrinogen alpha Chain Antibody, which binds the captured Fibrinogen alpha Chain present in each well. HRP-Avidin conjugate is then added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Fibrinogen alpha Chain captured in each well. The concentration of Fibrinogen alpha Chain can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Storage

Store kit components at +4°C or -20°C as instructed. Do not use past expiration date!

Documentație

• Manual de utilizare (PDF)se deschide pe site-ul producătorului