Lyme Disease Borrelia burgdorferi sensu lato IgM ELISA Kit

Pe scurt

Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

IgM

Applications

ELISA

Reactivity

B. burgdorferi sensu stricto, B. afzelii, B. garinii, B. mayonii. B. spielmanii. B. bissetiae, B. bavariensis and B. valaisiana

Isotype

IgM

Recommended dilutions

A 1:51 sample dilution is recommended.

Assay type

Indirect ELISA

Sensitivity

The assay shows a sensitivity of 76.7 percent in late Lyme disease and Lyme neuroborreliosis, 45 percent in Lyme arthritis, and 57.1 percent in Lyme carditis, and demonstrates greater than 87.8 percent sensitivity in acute Lyme disease within six weeks of infection, with observed sensitivities of 55.1 percent in acute Lyme disease and 59.6 percent in early Lyme disease.

Sample type

Serum and plasma

Detection type

HRP Colorimetric

Platform

Pre-coated Microplate (12 x 8 well strips)

Assay Time

1h 40m

Imunogen

This kit uses an immunogen composed of a mixture of 120 linear immunodominant epitopes together with 4 flagellin subunits, 12 full-length VlsE proteins, and 8 full-length OspC antigens, to capture IgM antibodies indicative of an early immune response to Borrelia.

Principiul testului

Lyme Disease Borrelia burgdorferi sensu lato IgM ELISA Kit employs an indirect ELISA for the quantitative measurement of IgM antibodies to Borrelia burgdorferi sensu stricto, B. afzelii, B. garinii, B. mayonii, B. spielmanii, B. bissetiae, B. bavariensis, and B. valaisiana in serum and plasma. The microplate wells are pre-coated with Borrelia antigens. Samples and standards are incubated in the wells to allow specific IgM antibodies to bind. After washing, a biotinylated detection antibody is added, followed by HRP-streptavidin. TMB substrate is then introduced, producing a colour change through HRP-mediated enzymatic activity. The reaction is stopped with acidic solution, and absorbance is measured at 450 nm. The optical density is directly proportional to the concentration of Borrelia-specific IgM in the sample and is quantified using a standard curve.

Storage

Shipped at 4°C. Store at 4°C.