Lyme Disease Borrelia burgdorferi sensu lato IgG + IgM ELISA Kit

Pe scurt

Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

IgM

Applications

ELISA

Reactivity

B. burgdorferi sensu stricto, B. afzelii, B. garinii, B. mayonii. B. spielmanii. B. bissetiae, B. bavariensis and B. valaisiana

Isotype

IgM

Recommended dilutions

A 1:51 sample dilution is recommended.

Assay type

Indirect ELISA

Sample type

Serum and plasma

Detection type

HRP Colorimetric

Platform

Pre-coated Microplate (12 x 8 well strips)

Assay Time

1h 40m

Imunogen

This kit uses a defined panel of 27 VlsE antigens corresponding to the main Borrelia species and subspecies as the immunogen, enabling specific detection of VlsE-directed IgM antibodies with enhanced diagnostic specificity.

Principiul testului

Lyme Disease Borrelia burgdorferi sensu lato IgG + IgM ELISA Kit employs an indirect ELISA for the quantitative measurement of IgG and IgM antibodies to Borrelia burgdorferi sensu stricto, B. afzelii, B. garinii, B. mayonii, B. spielmanii, B. bissetiae, B. bavariensis, and B. valaisiana in serum and plasma. The 96-well microplate is pre-coated with Borrelia antigens. Samples and standards are added and incubated, allowing specific antibodies to bind to the immobilized antigens. After washing, a biotinylated detection antibody is applied, followed by HRP-streptavidin. TMB substrate is added to generate a colourimetric reaction catalyzed by HRP. The reaction is stopped with acidic solution, and absorbance is read at 450 nm. The signal intensity is proportional to the concentration of Borrelia-specific antibodies present and is determined by comparison with a standard curve.

Storage

Shipped at 4°C. Store at 4°C.