Human VEGF165 ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

VEGF165

Applications

Sandwhich ELISA

Reactivity

Human

Conjugate

Unconjugated

Assay type

Sandwich (quantitative)

Sensitivity

15.9pg/ml

Sample type

Serum, plasma, and cell culture supernatants.

Cross reactivity

No cross reaction with VEGF-121, VEGF-145, VEGF-B, VEGF-C, VEGF-D, VEGF-R1, Ang-2. Shows cross reactivity with VEGF-189 isoform.

Detection type

Colorimetric

Platform

Pre-coated Microplate (1 x 96)

Product range

31.25-1000 pg/ml

Assay Time

3h 45m

Specificitate

This product recognizes human VEGF165 isoforms.

Principiul testului

Human VEGF165 ELISA Kit (A341783) is a sandwich ELISA designed for the sensitive and quantitative measurement of human VEGF165 in serum, plasma and cell culture supernatants. The assay utilizes a 96-well microtiter plate pre-coated with an antibody specific for VEGF165. Standards and samples are added to the wells, allowing VEGF165 present in the samples to bind to the immobilized capture antibody. After washing, a biotinylated detection antibody specific for VEGF165 is added, forming an antibody–antigen–antibody complex. Unbound detection antibody is removed by washing, followed by the addition of streptavidin conjugated to horseradish peroxidase (HRP). Following incubation and washing, TMB substrate is added, resulting in a colorimetric reaction that produces a blue color which turns yellow upon addition of stop solution. The intensity of the color is directly proportional to the concentration of VEGF165 in the sample. The concentration of VEGF165 is determined by measuring the absorbance at 450 nm and comparing the results to the standard curve.

Storage

Store at 4°C.