Human Angiopoietin 2 ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

Angiopoietin 2

Applications

Sandwhich ELISA

Reactivity

Human

Conjugate

Unconjugated

Assay type

Sandwich (quantitative)

Sensitivity

25 pg/ml

Sample type

Serum, plasma, and cell culture supernatants.

Cross reactivity

This product shows no cross reactivity with VEGF-A, GM-CSF, IL-34, IL-29 IFNg, IL-2, IL-10, CD138, MICB, MCP-1.

Detection type

Colorimetric

Platform

Pre-coated Microplate (1 x 96)

Product range

125-4000 pg/ml

Assay Time

3h 45m

Specificitate

This product recognizes natural and recombinant Angiopoietin-2.

Principiul testului

Human Angiopoietin 2 ELISA Kit (A341784) is a sandwich ELISA designed for the sensitive and quantitative measurement of human Angiopoietin 2 in serum, plasma and cell culture supernatants. The assay utilizes a 96-well microtiter plate pre-coated with an antibody specific for Angiopoietin 2. Standards and samples are added to the wells, allowing Angiopoietin 2 present in the samples to bind to the immobilized capture antibody. After washing, a biotinylated detection antibody specific for Angiopoietin 2 is added, forming an antibody–antigen–antibody complex. Unbound detection antibody is removed by washing, followed by the addition of streptavidin conjugated to horseradish peroxidase (HRP). Following incubation and washing, TMB substrate is added, resulting in a colorimetric reaction that produces a blue color which turns yellow upon addition of stop solution. The intensity of the color is directly proportional to the concentration of Angiopoietin 2 in the sample. The concentration of Angiopoietin 2 is determined by measuring the absorbance at 450 nm and comparing the results to the standard curve.

Storage

Store at 4°C.