Human Transforming Growth Factor beta 1 ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărimi disponibile

96T · 192T · 192T x 3

Țintă

Transforming growth factor beta 1

Applications

Sandwich ELISA

Reactivity

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 9 pg/ml

Sample type

Serum, plasma, cell culture supernatant, and other biological fluids.

Cross reactivity

This assay has shown no cross-reactivity with other cytokines tested.

Detection type

Colorimetric

Platform

Pre-coated Microplate (12 x 8 well strips)

Product range

9- 2500 pg/ml

Assay Time

3h 30m

Specificitate

This sandwich ELISA recognizes both natural and recombinant human TGF-ß1.

Principiul testului

This TGF-ß1 enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to TGF-ß1. Standards or samples are then added to the appropriate microtiter plate wells and incubated. After washing, biotin conjugated polyclonal antibody specific to TGF-ß1 is added and incubated. TGF-ß1 if present, will bind and become immobilized by the antibody pre-coated on the wells and then be “sandwiched” by biotin conjugate. The microtiter plate wells are thoroughly washed to remove unbound TGF-ß1 and other components of the sample. In order to quantitatively determine the amount of TGF-ß1 present in the sample, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Avidin is a tetramer containing four identical subunits that each has a high affinity-binding site for biotin. The wells are thoroughly washed to remove all unbound HRP-conjugated Avidin and a TMB (3,3'5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain TGF-ß1, biotin-conjugated antibody, and enzyme-conjugated Avidin will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. In order to measure the concentration of TGF-ß1 in the samples, this kit includes two calibration diluents (Calibrator Diluent I for serum/plasma testing and Calibrator Diluent II for cell culture supernatant testing.) According to the testing system, the provided standard is diluted (2-fold) with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D) versus TGF-ß1 concentration (pg/mL). The concentration of TGF- ß1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Storage

Shipped and stored at 4°C.