Human Interleukin-2 ELISA Kit

Pe scurt

Descriere

Specificații

Brand

Antibodies

Mărimi disponibile

96T · 192T · 192T x 3

Țintă

Interleukin-2

Applications

Sandwich ELISA

Reactivity

Human

Assay type

Sandwich (quantitative)

Sensitivity

6.7 pg/ml

Sample type

Serum, plasma, cell culture supernatant, and other biological fluids.

Cross reactivity

This assay has shown no cross-reactivity with other cytokines tested.

Detection type

Colorimetric

Platform

Pre-coated Microplate (12 x 8 well strips)

Product range

6.7-2500 pg/ml

Assay Time

4h 30m

Specificitate

This sandwich ELISA can detect both natural and recombinant human IL-2.

Principiul testului

This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-2 present is bound by the immobilized antibody. After washing away any unbound substances, an enzyme-linked polyclonal antibody specific for IL-2 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and colour develops in proportion to the amount of IL-2 bound in the initial step. The colour development is stopped and the intensity of the colour is measured. In order to measure the concentration of IL-2 in the samples this kit includes two calibration diluents (Calibrator Diluent I for serum/plasma testing and Calibrator Diluent II for cell culture supernatant testing.) According to the testing system, the provided standard is diluted (2-fold) with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D) versus IL-2 concentration (pg/mL). The concentration of IL-2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Storage

Shipped and stored at 4°C.