Human TNF alpha ELISpot Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

TNF Alpha

Applications

ELISpot

Reactivity

Human

Conjugate

Unconjugated

Sample type

Peripheral blood mononuclear cells (PBMCs).

Cross reactivity

This product shows no cross reactivity with other human cytokines. Cross reacivity with macac TNF-a. No interference with TNFR I and TNFR II.

Detection type

Colorimetric

Platform

Pre-coated Microplate (1 x 96)

Assay Time

3h after cell stimulation

Specificitate

This product recognizes natural human TNF-a.

Principiul testului

Human TNF alpha ELISpot Kit (A341810) is a sandwich ELISA designed for the sensitive and quantitative measurement of human TNF alpha in peripheral blood mononuclear cells (pbmcs). The assay utilizes a 96-well microtiter plate pre-coated with an antibody specific for TNF alpha. Standards and samples are added to the wells, allowing TNF alpha present in the samples to bind to the immobilized capture antibody. After washing, a biotinylated detection antibody specific for TNF alpha is added, forming an antibody–antigen–antibody complex. Unbound detection antibody is removed by washing, followed by the addition of streptavidin conjugated to horseradish peroxidase (HRP). Following incubation and washing, TMB substrate is added, resulting in a colorimetric reaction that produces a blue color which turns yellow upon addition of stop solution. The intensity of the color is directly proportional to the concentration of TNF alpha in the sample. The concentration of TNF alpha is determined by measuring the absorbance at 450 nm and comparing the results to the standard curve.

Storage

Store at 4°C.