Human TNF alpha ELISA Kit

Pe scurt

Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

TNF Alpha

Applications

Sandwhich ELISA

Reactivity

Human

Conjugate

Unconjugated

Assay type

Sandwich (quantitative)

Sensitivity

< 8 pg/ml

Sample type

Serum, plasma, and cell culture supernatants.

Cross reactivity

This product shows no cross reactivity with other human cytokines. Cross reactivity with macac TNF-a. No interference with soluble TNFR I and TNFR II.

Detection type

Colorimetric

Platform

Pre-coated Microplate (1 x 96)

Product range

25-800 pg/ml

Assay Time

3h 45m

Specificitate

This product recognizes both natural and recombinant human TNF-a.

Principiul testului

Human TNF alpha ELISA Kit (A341803) is a sandwich ELISA designed for the sensitive and quantitative measurement of human TNF alpha in serum, plasma and cell culture supernatants. The assay utilizes a 96-well microtiter plate pre-coated with an antibody specific for TNF alpha. Standards and samples are added to the wells, allowing TNF alpha present in the samples to bind to the immobilized capture antibody. After washing, a biotinylated detection antibody specific for TNF alpha is added, forming an antibody–antigen–antibody complex. Unbound detection antibody is removed by washing, followed by the addition of streptavidin conjugated to horseradish peroxidase (HRP). Following incubation and washing, TMB substrate is added, resulting in a colorimetric reaction that produces a blue color which turns yellow upon addition of stop solution. The intensity of the color is directly proportional to the concentration of TNF alpha in the sample. The concentration of TNF alpha is determined by measuring the absorbance at 450 nm and comparing the results to the standard curve.

Storage

Store at 4°C.