Human Thrombospondin-1 ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

Thrombospondin-1

Applications

Sandwich ELISA

Reactivity

Human

Assay type

Sandwich (quantitative)

Sample type

Plasma, serum, and lysates.

Cross reactivity

No reported cross-reactivity.

Detection type

Colorimetric

Platform

Pre-coated Microplate (12 x 8 well strips)

Product range

40-25000 pg/ml

Principiul testului

This Human Thrombospondin-1 ELISA Kit allows for the quantitative determination of natural or recombinant Thrombospondin-1 within any experimental sample including cell lysates, serum, and plasma. This Human Thrombospondin-1 ELISA Kit applies a technique called quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for Thrombospondin-1. Standards or samples are then added to the appropriate microtiter plate wells and incubated. If present, Thrombospondin-1 will bind and become immobilized by the antibody pre-coated on the wells. Thrombospondin-1 then becomes “sandwiched” by the primary capture antibodies and the secondary detection antibodies subsequently added. After incubation and “sandwiching” of Thrombospondin-1, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3’, 5, 5’-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.

Storage

Store at + 4°C. Stable for 3-6 months.