Imagine indisponibilăHuman Thrombospondin 1 ELISA Kit
Pe scurt
Human Thrombospondin 1 ELISA Kit este un kit ELISA de tip Sandwich (quantitative) pentru măsurarea Thrombospondin-1, cu reactivitate declarată pentru Human. Măsoară în domeniul 3.125-200 ng/ml, are o sensibilitate de 1.875 ng/ml, folosește detecție Colorimetric Detection (OD450) și se poate folosi pe probe de tip Serum, plasma, tissue homogenates, and other biological fluids. Se livrează în mărimea 96T, la prețul de 4.392,30 lei cu TVA.
Produse destinate exclusiv cercetării. Nu se utilizează în diagnostic sau tratament.
Descriere
Human Thrombospondin 1 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Thrombospondin 1 in serum, plasma, tissue homogenates, and other biological fluids.
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Specificații
- Brand
- Antibodies
- Mărime
- 96T
- Țintă
- Thrombospondin-1
- Applications
- Sandwich ELISA
- Reactivity
- Human
- Assay type
- Sandwich (quantitative)
- Sensitivity
- 1.875 ng/ml
- Cross reactivity
- Specific for Thrombospondin 1 with no reported cross-reactivity with other analogs or variants.
- Detection type
- Colorimetric Detection (OD450)
- Product range
- 3.125-200 ng/ml
- Assay Time
- 4 hours
- Principiul testului
- Human Thrombospondin 1 ELISA Kit (A75927) is a 4 hour sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Thrombospondin 1 in serum, plasma, tissue homogenates, and other biological fluids. A 96-well microtiter plate has been pre-coated with an antibody specific for Thrombospondin 1. Standards and samples are added to the wells, where Thrombospondin 1 binds to the immobilized capture antibody. Following incubation, unbound components are removed by washing and a biotinylated detection antibody specific for Thrombospondin 1 is added to the wells. The detection antibody binds the captured Thrombospondin 1 to form an antibody-antigen-antibody sandwich complex. After further incubation and washing, HRP-Streptavidin conjugate is added to the wells. TMB substrate solution is then added to visualize the HRP enzymatic reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is directly proportional to the amount of Thrombospondin 1 captured in each well. The concentration of Thrombospondin 1 is calculated by reading the absorbance at 450 nm and comparing the values with the standard curve.
Documentație
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