Human Serpin E1 ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

Serpin E1

Applications

Sandwich ELISA

Reactivity

Human

Assay type

Sandwich (quantitative)

Sensitivity

1.53 pg/ml

Sample type

Cell culture supernatant, serum and plasma.

Cross reactivity

This ELISA kit has shown no significant cross-reactivity.

Detection type

Colorimetric

Platform

Microplate

Product range

312.50 - 20000 pg/ml

Recovery

90% (Average Recovery from Serum Samples).

Specificitate

This kit can assay both native and recombinant human Serpin E1.

Principiul testului

Human Serpin E1 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human Serpin E1 in the samples. A monoclonal antibody specific for human Serpin E1 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for Serpin E1, and Serpin E1 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of Serpin E1 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Storage

Store at +4°C. Do not use past expiration date!