Human L Selectin ELISA Kit

Pe scurt

Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

L Selectin

Applications

Sandwich ELISA

Reactivity

Human

Assay type

Sandwich (quantitative)

Sensitivity

9.46 pg/ml

Sample type

Cell culture supernatant, serum and plasma.

Cross reactivity

This ELISA kit has shown no significant cross-reactivity.

Detection type

Colorimetric

Platform

Microplate

Product range

78.13 - 5000 pg/ml

Recovery

104% (Average Recovery from Serum Samples).

Specificitate

This kit can assay both native and recombinant human sL-Selectin.

Principiul testului

Human sL-Selectin ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human sL-Selectin in the samples. A monoclonal antibody specific for human sL-Selectin has been immobilized onto microwells. Standard or samples are pipetted into the wells, and sL-Selectin present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-linked monoclonal antibody specific for sL-Selectin is added to the wells. Following a wash to remove any unbound detect antibody, streptavidin-HRP is added. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of sL-Selectin bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Storage

Store at +4°C. Do not use past expiration date!