Human Interleukin-8 ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărimi disponibile

96T · 192T · 192T x 3

Țintă

Interleukin 8

Applications

Sandwich ELISA

Reactivity

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 10 pg/ml

Sample type

Serum, plasma and cell culture supernatant.

Cross reactivity

This assay has shown no detectable cross-reactivity with human monocyte chemotactic activating factor (MCAF) or RANTES (Regulated on Activation, Normal T-cell Expressed, and Secreted).

Detection type

Colorimetric

Platform

Pre-coated Microplate (12 x 8 well strips)

Product range

10-1600 pg/ml

Assay Time

2h 30m

Specificitate

This sandwich ELISA recognizes both natural and recombinant human IL-8.

Principiul testului

This IL-8 enzyme-linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for IL-8. Standards or samples are then added to the appropriate microtiter plate wells and incubated. IL-8, if present, will bind and become immobilized by the antibody pre-coated on the wells. The microtiter plate wells are thoroughly washed to remove unbound IL-8 and other components of the sample. In order to quantify the amount of IL-8 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated monoclonal antibody specific for IL-8 is added to each well to "sandwich" the IL-8 immobilized during the first incubation. The microtiter plate then undergoes a second incubation. The wells are thoroughly washed to remove all unbound HRP-conjugated antibodies and a TMB (3,3'5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IL-8 and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450nm ± 2nm. In order to measure the concentration of IL-8 in the samples, this kit contains two calibration diluents (Calibrator Diluent I for serum/plasma testing and Calibrator Diluent II for cell culture supernatant/ urine testing). According to the testing system, the provided standard is diluted (2-fold) with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D.) versus IL-8 concentration (pg/mL). The concentration of IL-8 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Storage

Shipped and stored at 4°C.