Human Prolactin ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

Prolactin

Applications

Sandwich ELISA

Reactivity

Human

Assay type

Sandwich (quantitative)

Sensitivity

1.5 ng/ml

Sample type

Serum.

Cross reactivity

This assay has shown no detectable cross-reactivity with human FSH, LH, and TSH, hCG and hGH.

Detection type

Colorimetric

Platform

Pre-coated Microplate (12 x 8 well strips)

Product range

1.5-200 ng/ml

Principiul testului

This Prolactin enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for Prolactin. Standards or samples are then added to the microtiter plate wells and Prolactin if present, will bind to the antibody pre-coated on the wells. In order to quantitate the amount of Prolactin present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated monoclonal antibody, specific for Prolactin are added to each well to “sandwich” the Prolactin immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain Prolactin and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm. In order to measure the concentration of Prolactin in the sample, this Human Prolactin ELISA Kit includes a set of calibration standards (6 standards). The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density (O.D.) versus Prolactin concentration (ng/mL). The concentration of Prolactin in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Storage

Shipped and stored at 4°C.