Human IFN gamma ELISpot Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

IFN gamma

Applications

ELISpot

Reactivity

Human

Host

Human

Assay type

sandwich ELISA

Sample type

Peripheral blood mononuclear cells (PBMCs) in single-cell suspension, capable of cytokine secretion.

Detection type

Colorimetric

Platform

Precoated PVDF Microplate

Assay Time

Assay time: 3 hours. Sample incubation time: 16-24 hours.

Specificitate

This ELISpot Kit specifically targets Human IFN gamma.

Principiul testului

The Human IFN gamma ELISpot Kit (A334653/A334654) employs the double-antibody sandwich ELISpot technique for the detection and quantification of IFN gamma-secreting cells at the single-cell level. A capture antibody specific for human IFN gamma is pre-coated onto a PVDF 96-well microplate (or detachable strip format). Test cells, such as peripheral blood mononuclear cells (PBMCs), are added into the wells and, upon stimulation, secrete IFN gamma that is captured by the immobilized antibody. A biotinylated detection antibody specific for IFN gamma is then added, which binds to the captured cytokine, followed by Streptavidin-HRP, which binds the biotinylated antibody. After incubation, unbound detection antibody and Streptavidin-HRP are removed by washing, and an AEC substrate solution is added. Spots develop at the sites of cytokine secretion in proportion to the number of IFN gamma-producing cells. The color development can be quantified using an ELISpot reader, providing the frequency of cytokine-secreting cells in the sample. This kit is ready-to-use, including pre-coated plates/strips, detection antibody, Streptavidin-HRP, AEC coloring system, dilution buffer, cell stimulant, and washing buffer.

Storage

Shipped at 4°C. The kit should be stored at 2-8°C. Shelf life is 12 months from the date of receipt.

Documentație

• Manual de utilizare (PDF)se deschide pe site-ul producătorului