Human IFN gamma ELISA Kit

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Descriere

Specificații

Brand

Antibodies

Mărime

96T

Țintă

IFN gamma

Applications

Sandwich ELISA

Reactivity

Human

Recommended dilutions

Validated sample dilutions include healthy serum (undiluted), healthy plasma (undiluted), LPS-stimulated human peripheral blood lymphocyte culture supernatants (1:10-1:25), PHA-stimulated human peripheral blood lymphocyte culture supernatants (undiluted), and PHA-stimulated human peripheral blood lymphocyte lysates (1:2). Sample dilution should be optimized according to the expected IFN gamma concentration.

Assay type

Sandwich (quantitative)

Sensitivity

9.375 pg/ml

Sample type

Serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids.

Cross reactivity

Specific for IFN gamma with no reported cross-reactivity with other analogs or variants.

Detection type

Colorimetric Detection (OD450)

Platform

Pre-coated Microplate (12 x 8 well strips)

Product range

15.625-1000 pg/ml

Assay Time

2 hours

Principiul testului

Human IFN gamma ELISA Kit (A335540) is a 2 hour sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human IFN gamma in serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids. This QuickRun format uses a pre-coated 96-well microtiter plate and optimized ready-to-use reagents to reduce assay time. Standards and samples are added to the wells, where IFN gamma binds to the immobilized capture antibody. Assay-specific biotinylated detection antibody and HRP-Streptavidin reagents are then added according to the optimized QuickRun workflow. These reagents form an antibody-antigen-antibody sandwich complex with the captured IFN gamma. TMB substrate solution is added to visualize the HRP enzymatic reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is directly proportional to the amount of IFN gamma captured in each well. The concentration of IFN gamma is calculated by reading the absorbance at 450 nm and comparing the values with the standard curve.

Storage

Shipped at +4°C. Store kit components at +4°C or -20°C as instructed in the Protocol Manual. Do not use past expiration date! Avoid freeze/thaw cycles.