Anti-Vimentin Antibody [VM452] (CF® 488)

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Descriere

Specificații

Brand

Antibodies

Mărime

100T

Țintă

Vimentin

Applications

Flow Cytometry, IF

Reactivity

Human, Bovine, Canine, Feline, Porcine, Goat, Chicken

Formulation

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Conjugate

CF®488

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

100 µg/ml

Purification

Protein A/G chromatography.

Isotype

IgG1

Recommended dilutions

Flow Cytometry: 5 µl / million cells or 5 µl / 100 µl whole blood, IF: 1:50-1:100

Molecular weight

57-60 kDa

Cross reactivity

This antibody does not cross-react with Mouse or Rat.

Light chain

kappa

Clonă

VM452

Imunogen

Recombinant human Vimentin protein.

Specificitate

This antibody reacts with a 58kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.

Storage

Store at 4-8°C. Antibody is stable for 24 months.

Documentație

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