Anti-Vimentin Antibody [rVIM/6575]

Pe scurt

Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

Vimentin

Applications

WB, IHC-P

Reactivity

Human, Rat, Horse, Chicken, Bovine, Feline, Canine, Porcine

Formulation

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Conjugate

Unconjugated

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

200 µg/ml

Purification

Protein A/G chromatography.

Isotype

IgG1

Recommended dilutions

WB: 1-2 µg/ml, IHC-P: 1-2 µg/ml

Molecular weight

57-60 kDa

Cross reactivity

This antibody does not cross-react with Mouse.

Light chain

kappa

Clonă

rVIM/6575

Imunogen

Recombinant full-length human Vimentin protein.

Specificitate

This antibody reacts with a 58kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP s) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.Anti-vimentin alone is of limited value as a diagnostic tool; however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Documentație

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