Anti-VEGFA Antibody - BSA and Azide free

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Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

VEGFA

Applications

WB, IHC-P, ELISA, Functional Assays

Reactivity

Human

Formulation

Supplied in Phosphate Buffered Saline.

Conjugate

Unconjugated

Host

Goat

Clonality

Polyclonal

Product form

Lyophilized

Concentration

1 mg/ml after reconstitution.

Isotype

IgG

Recommended dilutions

ELISA: 0.5 - 2 µg/ml, Functional Assays: 0.05 - 0.10 µg/ml, IHC-P: 5.0 - 15.0 µg/ml, WB: 0.1 - 0.2 µg/ml

Reconstitution

Reconstitute with 0.1 ml of distilled water. For long term storage the addition of 0.09% soidum azide is recommended. For functional studies do not add sodium azide.

Imunogen

Recombinant Human VEGF.

Specificitate

This antibody recognises human VEGF (vascular endothelial growth factor), a heparin-binding cytokine and member of the PDGF/VEGF growth factor family, which exists as several splice variants of the VEGF gene. VEGF, originally named vascular permeability factor due to its ability to act as vasodilator and increase microvascular permeability, is a potent stimulator of angiogenesis of both normal and cancerous cells, and acts as a regulator of vasculogenesis.The functional forms of VEGF exist as disulphide-linked homodimers and are classified according to the number of amino acid residues within a constitutive polypeptide chain e.g VEGF165. All VEGF family members act through binding to the tyrosine kinase receptors VEGFR-1 (Flt-1) and VEGFR-2 (KDR/CD309), whilst the closely-related VEGF-C and VEGF-D, can also bind to VEGFR-3 (Flt-4).

Storage

Lyophilized: Store at -20°C. Reconstituted: Store undiluted at -20°C, avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

Documentație

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