Anti-VEGFA Antibody [4B2-8] - BSA and Azide free

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Descriere

Specificații

Brand

Antibodies

Mărime

500µg

Țintă

VEGFA

Applications

WB, ELISA, Functional Assays

Reactivity

Human

Formulation

Supplied in Phosphate Buffered Saline.

Conjugate

Unconjugated

Host

Mouse

Clonality

Monoclonal

Product form

Lyophilized

Concentration

1 mg/ml after reconstitution.

Purification

Protein A affinity chromatography of tissue culture supernatant.

Isotype

IgG1

Recommended dilutions

ELISA: 2.0 - 4.0 µg/ml, Functional Assays: 0.1 - 0.2 µg/ml, WB: 0.25 - 0.50 µg/ml

Molecular weight

Approximately 38.2 kDa using recombinant human VEGF165 PHP091 under non-reducing conditions.

Reconstitution

Reconstitute with 0.5 ml of distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gently mix after reconstitution.

Clonă

4B2-8

Imunogen

Highly purified recombinant human VEGF165.

Specificitate

This antibody recognises human Vascular endothelial growth factor A , also known as VEGF-A or Vascular permeability factor. VEGF-A is a 206 amino acid ~45 kDa disulphide linked homodimeric heparin-binding cytokine and member of the PDGF/VEGF growth factor family, which exists as several splice variants of the VEGF gene, and is a potent stimulator of angiogenesis of both normal and cancerous cells, and acts as a regulator of vasculogenesis.The functional forms of VEGF are classified according to the number of amino acid residues within a constitutive polypeptide chain e.g VEGF165, and all VEGF family members act through binding to the tyrosine kinase receptors VEGFR-1 (Flt-1) and VEGFR-2 (KDR/CD309), whilst the closely-related VEGF-C and VEGF-D, can also bind to VEGFR-3 (Flt-4).

Storage

Lyophilized: Store at -20°C. Reconstituted: Store undiluted at -20°C, avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

Documentație

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