Anti-Topoisomerase II alpha Antibody [rTOP2A/6569]

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Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

Topoisomerase II alpha

Applications

WB, IHC-P

Reactivity

Human, Mouse

Formulation

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Conjugate

Unconjugated

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

200 µg/ml

Purification

Protein A/G chromatography.

Isotype

IgG2a

Recommended dilutions

WB: 1-2 µg/ml, IHC-P: 0.5-1 µg/ml

Molecular weight

170 kDa

Light chain

kappa

Clonă

rTOP2A/6569

Imunogen

Recombinant fragment, around amino acids 1,352-1,493, of human Topoisomerase II alpha. The exact sequence is proprietary.

Specificitate

It recognizes a 170kDa protein, which is identified as topoisomerase IIα. It shows no cross-reaction with Topoisomerase II β or I. Topo II α plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo II α is also implicated in drug resistance of tumor cells and has been shown to be over-expressed in many human cancers. Decreased expression of Topo IIa is the predominant mechanism of resistance to several chemotherapeutic agents.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Documentație

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