Anti-TNF alpha Antibody [P/T2] - Equivalent to Abcam (ab9579)

Pe scurt

Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

TNF Alpha

Applications

Flow Cytometry, IF, IHC-P

Reactivity

Human, Mouse, Rat, Rabbit, Feline, Canine, Zebrafish

Formulation

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Conjugate

Unconjugated

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

200 µg/ml

Isotype

IgM

Recommended dilutions

Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml, IHC-P: 2-4 µg/ml

Molecular weight

17 kDa

Light chain

kappa

Clonă

P/T2

Secvență

NGVELRDNQLVVPSEG

Imunogen

A hexadecapeptide corresponding to amino acids 115-130 of human TNF alpha, conjugated to thyroglobulin.

Specificitate

Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17kDa band following SDS PAGE under non-reducing conditions. TNF alpha is closely related to the 25kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Documentație

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