Anti-TNF alpha Antibody (Biotin) - BSA and Azide free

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Descriere

Specificații

Brand

Antibodies

Mărime

50µg

Țintă

TNF Alpha

Applications

WB, ELISA 

Reactivity

Human

Formulation

Supplied in Phosphate Buffered Saline.

Conjugate

Biotin

Host

Goat

Clonality

Polyclonal

Product form

Lyophilized

Concentration

100 µg/ml after reconstitution.

Isotype

IgG

Recommended dilutions

ELISA: 0.25 - 1 µg/ml, WB: 0.1 - 0.2 µg/ml

Reconstitution

Reconstitute with 0.5 ml of sterile Phosphate Buffered Saline containing 0.1% Bovine Serum Albumin. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gently mix after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.

Imunogen

Recombinant human TNF-alpha.

Specificitate

This antibody recognises human Tumour Necrosis Factor alpha (TNF–alpha), a 17.4kDa multi-functional pro-inflammatory cytokine primarily secreted by macrophages, but also produced by T and B lymphocytes, activated monocytes and fibroblasts.TNF-alpha exists in both a transmembrane and mature soluble form and has many biological properties, including activation of transcription factor NF-kappaB, the induction of apoptosis in various tumour cell lines and the stimulation of cell differentiation and proliferation under certain conditions. TNF-alpha also plays a role in inflammation, septic shock, autoimmune disease and rheumatoid arthritis.

Storage

Lyophilized: Store at -20°C. Reconstituted: Store undiluted at -20°C, avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

Documentație

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