Anti-TNF alpha Antibody - BSA and Azide free

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Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

TNF Alpha

Applications

IHC-Fr, WB, ELISA, Functional Assays

Reactivity

Mouse

Formulation

Supplied in Phosphate Buffered Saline.

Conjugate

Unconjugated

Host

Rabbit

Clonality

Polyclonal

Product form

Lyophilized

Concentration

1 mg/ml after reconstitution.

Isotype

IgG

Recommended dilutions

ELISA: 0.5 - 2 µg/ml, Functional Assays: 0.01 - 0.015 µg/ml, WB: 0.1 - 0.2 µg/ml

Reconstitution

Reconstitute with 0.1 ml of distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gently mix after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. For functional studies do not add sodium azide

Imunogen

Recombinant mouse TNF alpha.

Specificitate

This antibody recognises mouse Tumour Necrosis Factor alpha (TNF–alpha), a 17.3kDa multi-functional pro-inflammatory cytokine primarily secreted by macrophages, but also produced by T and B lymphocytes, activated monocytes and fibroblasts.TNF-alpha exists in both a transmembrane and mature soluble form and has many biological properties, including activation of transcription factor NF-KappaB, the induction of apoptosis in various tumour cell lines and the stimulation of cell differentiation and proliferation under certain conditions. TNF-alpha is involved in inflammation, septic shock, autoimmune disease and rheumatoid arthritis. This Rabbit anti Mouse TNF-alpha antibody can be used to neutralize the effects of mouse TNF-alpha.

Storage

Lyophilized: Store at -20°C. Reconstituted: Store undiluted at -20°C, avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

Documentație

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