Anti-TLR4 Antibody [HTA125] - BSA and Azide free

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Descriere

Specificații

Brand

Antibodies

Mărime

500µg

Țintă

TLR4

Applications

IP, Flow Cytometry, Functional Assays

Reactivity

Human, Rhesus Monkey, Guinea Pig, Pig, Dog, Bovine

Formulation

Supplied in Phosphate Buffered Saline.

Conjugate

Unconjugated

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

1 mg/ml

Purification

Protein A affinity chromatography of tissue culture supernatant.

Isotype

IgG2a

Recommended dilutions

Flow Cytometry: 1:10 - 1:50 Pack Size: 0.1 mg 1:25 Pack Size: 0.2 mg, Use 10µl of the suggested working dilution to label 10⁶ cells or 100µl whole blood

Clonă

HTA125

Imunogen

Ba/F3 cell line expressing TLR4 (CD284).

Specificitate

This antibody recognises the human Toll like receptor 4 (TLR4) cell surface antigen.TLR4, also known as CD284, has been demonstrated to act as a receptor for LPS on human monocytes and macrophages. TLR4 signalling of LPS stimulation requires the presence of the MD-2 molecule.TLR4 is weakly expressed by resting cells, but is upregulated following stimulation with LPS.This antibody has been demonstrated to block activation of monocytes with LPS. The use of a preservative free format of Mouse anti Human CD284 antibody, clone HTA125 (MCA2061EL ) is recommended for functional assays.

Storage

Store undiluted at -20°C only. Storage in frost free freezers is not recommended. Avoid freeze-thaw cycles. Should this product contain a precipitate we recommend microcentrifugation before use.

Documentație

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