Anti-Sialoadhesin / CD169 Antibody [ED3]

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Descriere

Specificații

Brand

Antibodies

Mărimi disponibile

100µg · 250µg

Țintă

Sialoadhesin/CD169

Applications

IHC-Fr, IF, Flow Cytometry, IP

Reactivity

Rat

Formulation

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Conjugate

Unconjugated

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

500 µg/ml

Purification

Protein A affinity chromatography of tissue culture supernatant.

Isotype

IgG2a

Recommended dilutions

Flow Cytometry: 1:500, IHC-Fr: 1:50 - 1:250, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl.

Clonă

ED3

Imunogen

Rat spleen cell homogenate.

Specificitate

This antibody recognises the rat CD169 cell surface antigen, a ~185 kDa molecule expressed by macrophages, predominately confined to lymphoid organs only. Monocytes and granulocytes are negative. No other cell types are positive. The most conspicious property of ED3 is it stains marginal zone macrophages and marginal metallophils in the spleen very strongly. Furthermore, macrophages in (auto-immune) diseased tissues express the ED3 antigen. In healthy tissue no expression occurs. CD169 is a receptor for glycoconjugates containing sialic acid.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Documentație

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