Anti-Sialoadhesin / CD169 Antibody [7-239] (PE)

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Descriere

Specificații

Brand

Antibodies

Mărime

100T

Țintă

Sialoadhesin/CD169

Applications

Flow Cytometry

Reactivity

Human

Formulation

Supplied in Phosphate Buffered Saline with 0.2% BSA and <0.1% Sodium Azide.

Conjugate

PE

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

Lot Specific

Purification

Protein A affinity chromatography of tissue culture supernatant.

Isotype

IgG1

Recommended dilutions

Flow Cytometry: Neat, Use 10µl of the undiluted reagent to label 1x10⁶ cells in 100µl

Purity

> 95% (by SDS-PAGE).

Clonă

7-239

Imunogen

Human rhinovirus 14 infected monocyte-derived dendritic cells.

Specificitate

This antibody recognises CD169 also known as Siglec-1 or Sialoadhesin, is a member of the Siglec family of proteins. It is expressed by subpopulations of macrophages and dendritic cells. Some subpopulations of macrophages express CD169 at a low level, but this expression can be upregulated upon induction by IFN-a (O’Neill et al. 2013). CD169+ cells are largely found in the lymph nodes, spleen, but are also present in smaller amounts in intestinal tracts, liver and bone marrow. The most characterized functions of CD169 are its roles in cell-cell interactions and phagocytosis of sialylated pathogens. CD169 has an approximate molecular weight of 185 kDa and recognises sialic acid-containing sugar chains. Structurally, it contains an extracellular domain containing 17 immunoglobulin-like domains and one v-set domain via which it binds its’ ligands. It also contains 16 C2-set domains which extend the binding site away from the surface of the cell. This extension helps bind granulocytes, B cells, erythrocytes and a subset of CD8 T cells. Increased expression of CD169 has been found to be associated with various conditions, including atherosclerosis, type I diabetes, chronic rejection and systemic sclerosis (Bornhöfft et al. 2018). Mouse anti Human CD169 clone 7-239 has been used in flow cytometry experiments to measure cell surface expression of CD169 upon cell stimulation with IFN-a.

Storage

Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light.

Documentație

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