Anti-PI-9 Antibody [7D8]

Pe scurt

Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

PI-9

Applications

WB, IHC-P, Flow Cytometry, IHC-Fr

Reactivity

Human

Formulation

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Conjugate

Unconjugated

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

1 mg/ml

Purification

Protein G affinity chromatography of tissue culture supernatant.

Isotype

IgG1

Recommended dilutions

FC 1: 1:10 - 1:100, IHC-P 2: 1:50, Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl.

Cross reactivity

This antibody does not cross react with Pig or Mouse

Clonă

7D8

Imunogen

Recombinant PI-9 produced in P. pastoris.

Specificitate

This antibody recognises human PI-9 (proteinase inhibitor 9), also known as SerpinB9, a ~42kDa intracellular nucleocytoplasmic serpin expressed in cytotoxic lymphocytes (CTLs), natural killer (NK) cells, monocyte-derived dendritic cells (DCs), and to a lesser extent in B cells and myeloid cells.Granzyme B (grB) is a serine protease highly expressed by CTLs and NK cells, which is endocytosed by virus-infected and malignant target cells. The subsequent release of grB from the endocytic vesicles into the cytoplasm of the target cells, triggers grB-mediated apoptosis, through cleavage of various cytoplasmic or nuclear proteins. PI-9, up-regulated in response to grB production and degranulation, has been identified as a potent inhibitor of Granzyme B-mediated apoptosis, providing a vital self-protection mechanism against the premature apoptosis of CTLs and NK cells by grB, which may escape into the cytoplasm of the effector cells themselves.Clone 7D8 has been reported to work in western blotting applications. Bio-Rad recommend the use of MCA2540GA for this purpose. Clone 7D8 is suitable for use in indirect Immunofluorescence.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Documentație

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