Anti-pan Cytokeratin Antibody [SPM115 + SPM116]

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Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

pan-Cytokeratin

Applications

Flow Cytometry, IF, WB, IHC-P

Reactivity

Human, Monkey, Bovine, Canine, Rabbit, Mouse, Rat, Chicken

Formulation

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Conjugate

Unconjugated

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

200 µg/ml

Purification

Protein A/G chromatography.

Isotype

IgG1

Recommended dilutions

Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml, WB: 1-2 µg/ml, IHC-P: 1-2 µg/ml

Molecular weight

67 kDa (CK1) / 64 kDa (CK3) / 59 kDa (CK4) / 58 kDa (CK5) / 56 kDa (CK6) / 52 kDa (CK8) / 56.5 kDa (CK10) / 50 kDa (CK14) / 50 kDa (CK15) / 48 kDa (CK16) / 40 kDa (CK19)

Light chain

kappa

Clonă

SPM115 + SPM116

Imunogen

Human epidermal keratin.

Specificitate

Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8); 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19). Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE-1/AE-3 is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It has been used to characterize the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and has shown high sensitivity in the recognition of epithelial cells and carcinomas.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Documentație

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