Anti-pan Cytokeratin Antibody [PCK/3150]

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Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

pan-Cytokeratin

Applications

Flow Cytometry, IF, IHC-P

Reactivity

Human, Canine, Feline

Formulation

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Conjugate

Unconjugated

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

200 µg/ml

Purification

Protein A/G chromatography.

Isotype

IgG2a

Recommended dilutions

Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml, IHC-P: 1-2 µg/ml

Molecular weight

40-67 kDa (Multiple)

Light chain

kappa

Clonă

PCK/3150

Imunogen

Crude cytokeratin extract prepared from RT-4 and MCF-7 cells.

Specificitate

This antibody demonstrates a broad spectrum of cytokeratin reactivity. In normal tissues, this antibody is reactive with most epithelial types, including: bile ducts and hepatocytes in liver, bladder epithelium, breast ducts, bronchial epithelium, endometrium, intestinal epithelium of stomach, duodenum, ileum, colon, rectum, pancreas, ovarian epithelium, pancreatic acini, pituitary acini, pneumocytes, prostate, thyroid, skin (positive on the basal layer and negative on the superficial layers of squamous epithelium), and apocrine and sweat glands. In tumors, this antibody is reactive with most carcinomas, including breast, transitional cell (TCC), renal cell (RCC), lung adenocarcinoma, lung small cell, lung squamous cell, endometrial, prostate, ovarian, hepatocellular (HCC), colorectal CA, stomach and thyroid. This antibody is negative in certain normal tissues, including: brain, lymphocytes, all cells of hematolymphoid origin, muscle, brain, nerves, endothelium, and in certain tumors including: most melanomas, sarcomas, lymphomas, primitive neuroectodermal tumors (PNET) / Ewings, and gastrointestinal stromal tumors (GIST). Positivity has been seen on some dendritic cells in lymph nodes, some endothelia, and some muscle cells.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Documentație

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