Anti-p53 Antibody [DO-7]

Pe scurt

Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

p53

Applications

WB, IHC-P, IHC-Fr, Flow Cytometry, IP

Reactivity

Human, Bovine

Formulation

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Conjugate

Unconjugated

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

1 mg/ml

Purification

Protein A affinity chromatography of tissue culture supernatant.

Isotype

IgG2b

Recommended dilutions

FC 1: 1:50 - 1:100, IHC-P 2: 1:1,000, WB: 1:1,000, Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl

Cross reactivity

This antibody does not cross-react with Mouse or Rat.

Clonă

DO-7

Imunogen

Recombinant human p53.

Specificitate

This antibody recognises the human 53 kDa p53 tumour suppressor protein, also known as Cellular tumor antigen p53 or Antigen NY-CO-13, encoded by the TP53 gene. p53 is a 393 amino acid protein with an N-terminal transactivation domain, followed by a proline-rich region and a DNA binding domain in the central core region. The C-terminal region contains a tetramirization domain and a terminal regulatory domain. p53 is intimately involved in a number of signaling pathways controlling cell division, cycling and apoptosis and is thus a potent cancer suppressor. In normal cells the level of p53 expression is low but can be induced by DNA damage or other stress signals. Activation of p53 leads to growth arrest through its interaction with p21, GADD45 and 14-3-3s, DNA repair and potentially apoptosis through interaction with Bax, Apaf-1, PUMA and NoxA. p53 is critically regulated by Mdm2 which can trigger p53 degradation by a ubiquitin dependent system recognises an epitope at the N-terminal end of p53 between amino acids 20-25, binding to both wild type and mutant forms. Clone DO-7 is not expected to recognize the multiple isoforms lacking the N-teminal region.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Documentație

• Fișă tehnică (PDF)se deschide pe site-ul producătorului