Anti-p53 Antibody [DO-11]

Pe scurt

Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

p53

Applications

WB, IHC-P, IHC-Fr, IP

Reactivity

Human

Formulation

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Conjugate

Unconjugated

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

1 mg/ml

Purification

Protein A affinity chromatography of tissue culture supernatant.

Isotype

IgG1

Recommended dilutions

IHC-P 1: 1:1,000, WB: 1:200 - 1:2,000

Clonă

DO-11

Imunogen

Recombinant human p53.

Specificitate

This antibody recognises the human Cellular tumor antigen p53, also known as p53 tumor suppressor protein or NY-CO-13. p53 is a 393 amino acid ~53kDa cytoplasmic/ nuclear protein up-regulated in response to DNA damage and is found in a wide variety of transformed cells (UniProt: P04637).DO-11 binds to an epitope within the central region of p53 between amino acids 181 - 190 defining a cryptic epitope exposed in unfolded/ denatured p53. Nine isoforms of human p53 are produced by alternative splicing and promotor usage, the epitope recognized by clone DO-11 is present in all isoforms. recognises mutant forms of p53 and has been used successfully for detection of p53 by western blotting.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Documentație

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