Anti-MAP1LC3A + MAP1LC3B Antibody

Pe scurt

Descriere

Specificații

Brand

Antibodies

Mărimi disponibile

25µg · 100µg

Țintă

MAP1LC3A + MAP1LC3B

Applications

WB, IF 

Reactivity

Human, Mouse

Formulation

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Conjugate

Unconjugated

Host

Rabbit

Clonality

Polyclonal

Product form

Liquid

Concentration

1 mg/ml

Isotype

IgG

Recommended dilutions

WB: 1:1,000-1:5,000

Molecular weight

Approximately 14-15 kDa corresponding to LC3-II, and a band of approximately 17 kDa corresponding to LC3-I, in HeLa cell lysates.

Secvență

PSDRPFKQRRSFADC

Imunogen

Synthetic peptide sequence PSDRPFKQRRSFADC from the N-terminus of LC3A (NP_115903.1; NP_852610.1).

Specificitate

This antibody recognises an epitope within the N-terminal region of both MAP1LC3A (Microtubule-associated proteins 1A/1B light chain 3A/LC3A) and MAP1LC3B (Microtubule-associated proteins 1A/1B light chain 3B/LC3B), ubiquitin-like proteins and members of the MAP1LC3 family, which are widely used as reliable markers for the monitoring of autophagy.LC3-I is the cytosolic form of LC3, which is converted into the active, membrane-bound form LC3-II, during the autophagy process. Tracking the level of conversion of LC3-I to LC3-II provides an indicator of autophagic activity, and levels of LC3-II in particular, correlate with the extent of autophagosome formation, due to its association with the autophagosome membrane.recognises both the LC3-I and LC3-II forms of MAP1LC3A and MAP1LC3B.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Documentație

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