Anti-Macrophages/Monocytes/Granulocytes Antibody [MAC387] (APC)

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Descriere

Specificații

Brand

Antibodies

Mărime

100T

Țintă

Macrophages/Monocytes/Granulocytes

Applications

Flow Cytometry

Reactivity

Human, Horse, Pig, Dog, Rabbit, Baboon, Bovine, Guinea Pig, Rat, Cat, Cynomolgus monkey, Rhesus Monkey, Goat, Fallow deer, Pygmy hippopotamus, Mink, Marmoset

Formulation

Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.

Conjugate

APC

Host

Mouse

Clonality

Monoclonal

Product form

Lyophilized

Concentration

Reconstitution dependent.

Purification

Protein A affinity chromatography of tissue culture supernatant.

Isotype

IgG1

Recommended dilutions

FC 1: Neat - 1:5, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl

Reconstitution

Reconstitute with 1 ml of distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gently mix after reconstitution.

Clonă

MAC387

Imunogen

Human monocytes.

Specificitate

This antibody recognises the L1 or Calprotectin molecule, an intracytoplasmic antigen comprised of a 12 kDa alpha chain and a 14 kDa beta chain. Although originally described as binding to epitopes common to both the alpha and beta chains subsequent evidence indicates that the antibody detects an epitope exclusively expressed on the beta chain demonstrated by immunofluorescent and western blotting on both naturally expressing and transfected targets. In addition, Mouse anti Human macrophages, clone MAC387 detects the beta chain in complex with the alpha.The antigen recognized by Mouse anti Human macrophages, clone MAC387 is expressed by granulocytes, monocytes and by tissue macrophages. Variable results have been reported for staining brain macrophages and microglia. The epitope recognized appears to be well conserved and the antibody is routinely used for the detection of myeloid cells in a wide range of species.

Storage

Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

Documentație

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