Anti-CD45 Antibody [MAC323]

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Descriere

Specificații

Brand

Antibodies

Mărime

100µg

Țintă

CD45

Applications

IHC-P, IHC-Fr, Flow Cytometry, IP, IHC-R

Reactivity

Porcine

Formulation

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Conjugate

Unconjugated

Host

Mouse

Clonality

Monoclonal

Product form

Liquid

Concentration

1 mg/ml

Purification

Protein A affinity chromatography of tissue culture supernatant.

Isotype

IgG2a

Recommended dilutions

Flow Cytometry: 1:25 - 1:100, IHC-Fr: 1:10, IHC-P: 1:10, IHC-R 1: 1:10

Clonă

MAC323

Imunogen

Porcine Peripheral Blood Lymphocytes.

Specificitate

This antibody recognises an allotypic determinant of porcine CD45, also known as the leucocyte common antigen (LCA). recognises a monomorphic determinant on porcine CD45. When K252.1E4 is used in conjunction with MAC323, it can be shown that MAC323 stains a subset of the leukocytes that are detected by K252.1E4; indicating a loss of the epitope in MAC323-ve animals rather than loss of the CD45 antigen. Cross-breeding positive vs. negative animals indicate that the MAC323 gene is inherited in a simple Mendelian autosomal dominant pattern.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Documentație

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